| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,810,107 | +A | coding (25/1173 nt) | emrA → | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,810,104 | 1 | . | A | 100.0% | 11.4 / NA | 5 | Q8K (CAA→AAA) | emrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/5); total (0/5) | |||||||||||
CGCAAATGCGGAGACTC‑AAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTG > W3110S.gb/2810088‑2810151 | cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1665949/65‑1 (MQ=255)cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1791053/65‑1 (MQ=255)cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1797274/65‑1 (MQ=255)cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:627388/65‑1 (MQ=255)cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:922884/65‑1 (MQ=255) | CGCAAATGCGGAGACTC‑AAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTG > W3110S.gb/2810088‑2810151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |