Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,910,427	+C	coding (1881/2235 nt)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,910,423	1	.	C	100.0%	43.1 / NA	13	R629G (CGC→GGC)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (13/0);  total (13/0)

CGGTGTACTGAAATACCGCG‑CCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCT  >  W3110S.gb/2910404‑2910467                     |                                             cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAGGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:1056096/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAg                              >  1:1063699/1‑37 (MQ=38) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCg                           >  1:1603007/1‑40 (MQ=38) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGAc                         >  1:936887/1‑42 (MQ=38) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAatctcat                 >  1:1873223/1‑50 (MQ=39) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAat                      >  1:746241/1‑45 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:1024930/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:1085304/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:156049/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:1583248/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:166479/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:242927/1‑65 (MQ=255) cgGTGTACTGAAATACCGCGCCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCt  >  1:855596/1‑65 (MQ=255)                     |                                             CGGTGTACTGAAATACCGCG‑CCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCT  >  W3110S.gb/2910404‑2910467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑