Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,411,012	+A	coding (878/966 nt)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,411,009	1	.	A	81.8%	26.6 / 2.1	11	coding (875/966 nt)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (0/9);  total (2/9)
Fisher's exact test for biased strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGACCAGTTTCGGCGCACATTCAACGCCAT  >  W3110S.gb/3410960‑3411042                                                   |                                  tACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGACCACGCTCC‑AAATGACCAGTTTCg                    >  1:766237/1‑65 (MQ=255) tACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGCCCAGTTTCg                    >  1:1014999/1‑65 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1065523/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:10914/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1398566/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1412252/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:146183/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1491750/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:237714/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:520488/65‑1 (MQ=255)                    acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:916259/65‑1 (MQ=255)                                                   |                                  TACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGACCAGTTTCGGCGCACATTCAACGCCAT  >  W3110S.gb/3410960‑3411042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑