Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	494,792	+A	coding (449/1875 nt)	htpG →	molecular chaperone HSP90 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	494,788	1	.	A	100.0%	337.7 / NA	89	E149K (GAA→AAA)	htpG	molecular chaperone HSP90 family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/89);  total (0/89)

TGCGTACTCGCGCGGCAGGCGAAAAACCAG‑AAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGG  >  W3110S.gb/494759‑494822                               |                                   ttcGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:701305/63‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:453300/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:602913/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:60/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:584973/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:557376/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:549497/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:539437/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:513063/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:499635/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:498479/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:455639/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:641561/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:437401/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:430811/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:412750/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:37058/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:355540/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:345927/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:289609/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:265795/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:257648/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:239922/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:80032/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:992167/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:988532/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:974883/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:960997/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:921959/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:917174/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:827654/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:813040/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:808384/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:806511/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:103267/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:794715/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:791452/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:780290/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:771476/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:745463/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:727407/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:704907/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:694304/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:682683/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1297772/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1572294/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1535931/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1530057/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1513653/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1477137/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1471091/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1464656/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1428082/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1416742/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1344690/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:216931/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1242353/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1210327/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:117859/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1174244/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1161641/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1147582/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1088962/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1071894/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1068052/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1025190/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1597668/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:224078/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:194398/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1838952/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1834380/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1832976/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1784114/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1783538/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:177892/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1760626/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1756363/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1735295/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1715224/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1710327/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1673833/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1652776/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1646121/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1634657/65‑1 (MQ=255) tGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1576099/65‑1 (MQ=255)  gCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:113376/64‑1 (MQ=255)  gCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:1621731/64‑1 (MQ=255)  gCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:995674/64‑1 (MQ=255)                    cGAAAAACCAGAAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAgg  <  1:205475/46‑1 (MQ=38)                               |                                   TGCGTACTCGCGCGGCAGGCGAAAAACCAG‑AAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGG  >  W3110S.gb/494759‑494822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑