Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,036,204	(T)7→8	coding (31/1494 nt)	ftsY →	fused Signal Recognition Particle (SRP) receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,036,197	1	.	T	100.0%	68.5 / NA	20	G8G (GGC→GGT)	ftsY	fused Signal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/20);  total (0/20)

GTAGTCGCAAATGGCGAAAGAAAAAAAACGTGGC‑TTTTTTTCCTGGCTGGGCTTTGGTCAAAA  >  W3110S.gb/4036164‑4036226                                   |                              gtAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:26043/64‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:978999/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1134352/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:932311/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:832472/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:731786/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:727922/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:694699/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:564836/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:361656/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1701447/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1628363/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1594484/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1421361/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1295454/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1294139/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1213087/63‑1 (MQ=255)  tAGTCGCAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1150144/63‑1 (MQ=255)  tAGTCGAAAATGGCGAAAGAAAAAAAACGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:481000/63‑1 (MQ=255)                           aaCGTGGCTTTTTTTTCCTGGCTGGGCTTTGGTCaaaa  <  1:1400337/38‑1 (MQ=37)                                   |                              GTAGTCGCAAATGGCGAAAGAAAAAAAACGTGGC‑TTTTTTTCCTGGCTGGGCTTTGGTCAAAA  >  W3110S.gb/4036164‑4036226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑