Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,219,189 +T coding (120/1602 nt) aceB → malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,219,1851.T99.4% 644.5 / ‑3.4 169coding (116/1602 nt)aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (168/0);  total (169/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 170 total aligned reads displayed.

CTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCA‑TTTTACGCCACAACGCAATAAACT  >  W3110S.gb/4219146‑4219209
                                        |                        
cttccGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:242568/4‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGcc                 >  1:1376046/1‑50 (MQ=39)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCTATAAACt  >  1:1139656/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATaa     >  1:191216/1‑62 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAAc   >  1:1562203/1‑64 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:266690/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:530004/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:527288/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:515402/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:45930/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:392079/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:390308/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:38278/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:35590/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:331095/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:292471/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:281937/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:574023/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:265218/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:257918/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:249461/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:217884/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:214195/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:213514/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:198924/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:190515/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1855854/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:775913/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:961131/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:917364/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:914247/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:910367/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:897168/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:892903/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:87596/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:873136/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:843978/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:8355/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:815167/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1806249/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:774826/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:764241/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:728098/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:717993/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:71486/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:702890/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:685411/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:67805/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:641278/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:592192/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:586532/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1152599/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1811149/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1334097/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1329404/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1314441/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:13001/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1297157/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1277622/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1266943/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1194840/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:118214/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1168315/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1365757/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1128283/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1125728/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1111685/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1099475/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1076676/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1068630/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1051038/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:102553/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1022986/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:100513/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1001239/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1596850/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1000071/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:179068/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1775235/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:175435/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1735642/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1714093/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1694427/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1657138/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:163434/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1607243/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1378937/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1575997/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1555288/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1543885/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1536676/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1519471/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1470725/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1457419/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1457113/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1412096/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCA‑ttttta                    >  1:1628376/1‑44 (MQ=255)
cTGCCGAAGCGGTAGAATTTCTGACTGAGCTCGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1188049/1‑65 (MQ=255)
cTGCCGAAGCGGTAGAATTCCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:951784/1‑65 (MQ=255)
cTGCAGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTTACGCCACAACGCAATAAACt  >  1:1447631/1‑65 (MQ=255)
                                        |                        
CTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCA‑TTTTACGCCACAACGCAATAAACT  >  W3110S.gb/4219146‑4219209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑