Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3684687	3684731	45	2 [1]	[0] 14	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

CCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGC  >  W3110S.gb/3684732‑3684796 |                                                                 ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTg   >  1:1358587/1‑64 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1039094/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1064793/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1146292/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1183890/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1625451/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1702791/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:241083/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:520339/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:693317/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:816392/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:961534/1‑65 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACAGCTTg   >  1:487319/1‑64 (MQ=255) ccATTGTCGAACCATCCCTAACCGTTAGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGc  >  1:1589619/1‑65 (MQ=255) |                                                                 CCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAACGGTAACTGCTTGC  >  W3110S.gb/3684732‑3684796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑