Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4081822	4081845	24	133 [0]	[0] 6	rtcR	sigma 54‑dependent transcriptional regulator of rtcBA expression

TGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGTT  >  W3110S.gb/4081846‑4081910 |                                                                 tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:1240448/65‑1 (MQ=255) tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:1439502/65‑1 (MQ=255) tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:1478526/65‑1 (MQ=255) tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:1650448/65‑1 (MQ=255) tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:581047/65‑1 (MQ=255) tGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGtt  <  1:751744/65‑1 (MQ=255) |                                                                 TGAAACTCGTAACCACGGGCGAAATCATGCAGGCAGGCGTAGACCTCTTCGAAATCCCACGGGTT  >  W3110S.gb/4081846‑4081910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑