Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	453584	453713	130	19 [0]	[0] 44	[bolA]	[bolA]

ATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCG  >  W3110S.gb/453714‑453778 |                                                                 aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:677062/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:10562/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:460669/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:462338/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:514087/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:523474/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:524903/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:593968/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:608229/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:622960/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:652007/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:373753/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:684702/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:687638/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:776766/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:813606/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:828184/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:853802/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:893245/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:89965/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:932438/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:997204/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1443046/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1079099/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1123719/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:116003/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:11968/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1203275/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1289092/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1350280/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:140422/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:14312/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:315112/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1461037/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1512955/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1554089/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1654335/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1663254/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1726768/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1748603/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1835746/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:278075/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTTCCAACCAGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:1434748/65‑1 (MQ=255) aTAGAAGAAAAATTAAGGGCGGCGTGCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg  <  1:594751/65‑1 (MQ=255) |                                                                 ATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCG  >  W3110S.gb/453714‑453778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑