Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,251,8031.A36.4% 129.8 / 191.7 118coding (613/1071 nt)dhaKdihydroxyacetone kinase, N‑terminal domain
Reads supporting (aligned to +/- strand):  ref base . (75/0);  new base A (43/0);  total (118/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

ACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTG  >  W3110S.gb/1251750‑1251859
                                                      |                                                        
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAgg                                                 >  1:1793062/1‑64 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAgg                                                 >  1:121728/1‑64 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:388538/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:14380/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1558517/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:155946/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:158301/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1632690/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:169572/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:185809/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:211526/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:344588/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:350980/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:352300/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1005888/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:50455/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:538435/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:541184/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:549462/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:558107/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:695841/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:729776/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:808832/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:828201/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:858880/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:903523/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:908519/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:944523/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1198994/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1146462/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1383214/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1098557/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1298799/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAAGAAGGt                                                >  1:1117064/1‑65 (MQ=255)
aCCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTaaaa                                                      >  1:841204/1‑58 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCgg                                      >  1:1030123/1‑35 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCa                     >  1:1379232/1‑52 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCTCCGAGAGCGATa         >  1:302349/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGa           >  1:415095/1‑62 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGAt          >  1:1002669/1‑63 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:315056/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:270245/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1273367/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:437206/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1409576/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:51866/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1194046/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1177559/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:550132/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:577944/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:636764/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:110634/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:800513/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:975861/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:253247/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1339619/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1754895/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1796/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:183514/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1595478/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1504738/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1841281/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:1450172/1‑64 (MQ=255)
                                       aTTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATa         >  1:26921/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACa                         >  1:1371305/1‑42 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGc             >  1:1323732/1‑54 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGa           >  1:507166/1‑56 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:833011/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:1560770/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:1012667/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:1350593/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:1407272/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTAtt   >  1:332475/1‑64 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1378844/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:203121/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1404732/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1014917/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:903024/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1025502/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:342541/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1330534/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1214241/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:360354/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1567032/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:735074/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1577838/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:497711/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:69034/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1587399/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:608769/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:600117/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:58599/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:121086/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:569506/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:405880/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1681234/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:116690/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:45964/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:491566/1‑65 (MQ=255)
                                             cGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTg  >  1:1765401/1‑65 (MQ=255)
                                                      |                                                        
ACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTG  >  W3110S.gb/1251750‑1251859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: