| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,534,116 | 1 | . | T | 57.1% | 20.7 / 19.9 | 14 | coding (1507/2016 nt) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base T (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAGCGCGTT > W3110S.gb/2534081‑2534131 | tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:104682/47‑1 (MQ=255)tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:1329687/47‑1 (MQ=255)tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:1867541/47‑1 (MQ=255)tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:187833/47‑1 (MQ=255)tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:495858/47‑1 (MQ=255)tGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAgcg < 1:609242/47‑1 (MQ=255) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:1153904/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:124329/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:139489/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:1559110/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:183234/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:529467/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGtt > 1:736253/1‑48 (MQ=38) tAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGCTTTTTTCCAGCGCGt > 1:612271/1‑47 (MQ=38) | TGCATAGAGGAAGCGAGCAAAGGTGGTTTCTTTCGC‑TTTTTCCAGCGCGTT > W3110S.gb/2534081‑2534131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |