| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,348,578 | 0 | C | . | 47.5% | 268.7 / 221.9 | 122 | coding (272/633 nt) | yrbL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (24/40); new base . (58/0); total (82/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 122 total aligned reads displayed.
GATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACGA > W3110S.gb/3348552‑3348617 | gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGtt > 1:265101/1‑62 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGt > 1:835068/1‑61 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1430351/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1370937/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1309546/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:644271/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:688245/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:503263/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1195502/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:490989/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1141089/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1140912/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:756258/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:844609/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:884352/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1051118/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:445011/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:230798/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:987976/1‑63 (MQ=255)gATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTa > 1:1814871/1‑63 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTActa < 1:1743998/65‑3 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTAcaa < 1:1793226/65‑3 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:329965/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:259640/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:989880/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:209605/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:217368/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:961161/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:944066/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:268815/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:519189/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:402842/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:426149/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:92323/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:808090/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:745394/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:727928/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:555853/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:151793/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1096178/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:107292/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1048272/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1040868/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1613311/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1194511/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:163191/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1264491/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1502318/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1367965/65‑1 (MQ=255) aTTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:1703118/65‑1 (MQ=255) ttGCAGATTTTGTCGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1804495/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGaa > 1:1794430/1‑40 (MQ=39) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1129999/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:742501/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:630415/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1213425/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:700355/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1237307/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:672182/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:63558/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1739042/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:790604/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:801830/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1116941/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:837456/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:10947/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:947823/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:948559/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1023032/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:969812/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:975672/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1011337/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1346837/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1703623/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1675936/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1652581/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1838477/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1870776/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1627543/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1006387/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1557574/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1551668/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:270760/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1529303/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:360518/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:367611/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:44558/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:446353/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:45660/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:469064/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:476858/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:479866/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:489780/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1416488/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:1394570/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga > 1:593456/1‑63 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACg > 1:1488050/1‑62 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACg > 1:286741/1‑62 (MQ=255) ttGCAGATTTTGACGGCAAGCCTT‑CATTACGCTGACCGAATTTGCTGAACAATGCCGTTACg > 1:1009270/1‑62 (MQ=255) cAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACga < 1:780917/48‑1 (MQ=255) | GATTGCAGATTTTGACGGCAAGCCTTCCATTACGCTGACCGAATTTGCTGAACAATGCCGTTACGA > W3110S.gb/3348552‑3348617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |