Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,664,8180CA20.4% 128.5 / 19.9 44V202F (GTC→TTC) rffDUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (35/0);  new base A (9/0);  total (44/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTC  >  W3110S.gb/3664763‑3664827
                                                       |          
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTgac            >  1:1116678/1‑56 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACt   >  1:861239/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:584668/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1637019/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1037455/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1862189/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:272158/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:290409/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:303244/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:355482/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:505444/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:53868/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1664718/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:78429/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:808931/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:846772/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:852243/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:861904/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:908762/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:985303/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1394113/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1116084/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1137639/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1147968/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1160355/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1168943/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1182816/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1232048/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1247563/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1307944/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1381503/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1606504/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1434054/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1483649/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1502472/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTc  >  1:1569249/1‑65 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:49438/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:404130/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:672000/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:708953/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:1440598/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:1482853/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:286773/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:1497594/1‑64 (MQ=255)
tCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGAAGAGcacacg   >  1:181911/1‑64 (MQ=255)
                                                       |          
TCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGA‑CACACTC  >  W3110S.gb/3664763‑3664827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑