| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,689,445 | 0 | G | A | 30.2% | 124.9 / 73.8 | 86 | D244N (GAT→AAT) | hdfR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/60); new base A (26/0); total (26/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAT > W3110S.gb/3689390‑3689474 | cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:198570/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:129055/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:138905/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1395843/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1425463/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:152269/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1568335/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1705634/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1775090/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1875957/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:197307/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:226459/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1185021/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1173165/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:289207/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:29852/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:70492/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1151035/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:895689/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:960155/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcacac > 1:1020400/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcaca > 1:1832097/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcaca > 1:1302103/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcaca > 1:1245531/1‑64 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCAAGAGcac > 1:285112/1‑63 (MQ=255)cACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGG‑GGCCTGCATACCGTTGTCAAGAGcacacg > 1:973401/1‑63 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:225914/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:976043/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1191043/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:238767/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:274063/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1172521/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:297796/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:115554/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:314428/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:40909/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:443211/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:544521/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:545051/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:546904/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:548362/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:557821/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:60471/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:695497/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:716826/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:723033/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1133277/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:90537/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1087387/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:968623/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1225835/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1314709/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1261536/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1246118/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1410892/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1433867/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1454614/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1480157/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1495355/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1524920/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:204103/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1576348/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1584032/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1219388/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1191978/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1195731/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1885584/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1806985/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:181993/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1878439/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:119788/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCATTAt < 1:783110/65‑1 (MQ=255) gggCGCGTAAAAAAGGCGGCCTGAATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:622136/65‑1 (MQ=255) ggCGCGTAAAAACGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:452954/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1225431/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1384944/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1245938/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1016088/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:349963/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:365587/64‑1 (MQ=255) ggCGCGTAAAAAAGGCGGCCGGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:3300/64‑1 (MQ=255) gcgcGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:138606/63‑1 (MQ=255) ggcCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:774016/48‑1 (MQ=255) ccTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:856310/46‑1 (MQ=255) gCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:400211/43‑1 (MQ=255) tACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAt < 1:1151928/40‑1 (MQ=255) | CACCTGGCTACCCGTCAGCTGGGCGCGTAAAAAAGGCGGCCTGCATACCGTTGTCGATAGCACAACACTTTCACGGCCGCTTTAT > W3110S.gb/3689390‑3689474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |