| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 715,631 | 2 | . | A | 29.5% | 199.3 / 127.5 | 105 | intergenic (+11/‑203) | pgm/ybfP | phosphoglucomutase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/74); new base A (0/31); total (0/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 105 total aligned reads displayed.
GAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCTTTTT > W3110S.gb/715600‑715663 | gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:197155/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:477340/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:452834/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:433639/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:389695/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:333343/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:277051/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:263498/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:234943/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:478974/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1878954/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1828072/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1815558/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1783197/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1768129/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1745606/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1738721/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:665539/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:9394/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:915077/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:878034/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:861681/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:837720/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:819773/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:811597/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:71182/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1031698/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:636110/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:624100/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:594054/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:549962/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:499475/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:497537/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:496660/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1685851/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1104981/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1112494/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1125210/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1128696/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1130962/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1133690/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1145330/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1165425/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1228732/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:126998/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:133150/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1340613/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1544997/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:158311/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1630119/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1711647/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:166935/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1653294/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttc < 1:1491466/64‑2 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:1842852/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:73367/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:79672/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:331079/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:874933/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:1467145/64‑1 (MQ=255)gAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:248476/64‑1 (MQ=255)gAAGTGCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:124986/64‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:823817/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:740034/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1063677/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1022397/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:11584/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:246874/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:453997/65‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:634847/63‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:648344/63‑1 (MQ=255) aaGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:652266/63‑1 (MQ=255) aaGTCCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1563571/63‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:697995/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:315217/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:315768/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1086552/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1456705/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1780769/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:727593/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:196422/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1318346/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1837958/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:585802/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:580016/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:262050/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:935194/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AACGGCGGTCGCAAGATCGCCCttttt < 1:33346/64‑1 (MQ=255) aGTTCTGAAAAACGCGTAAACACAATTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1415118/64‑1 (MQ=255) tCTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1483241/61‑1 (MQ=255) cTGAAAAACGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:329602/60‑1 (MQ=255) cTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGGCGCAAGATCGCCCttttt < 1:1222727/58‑1 (MQ=255) aaaaaCGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:118450/57‑1 (MQ=38) aaaaaCGCGTAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:901820/57‑1 (MQ=38) aaCGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:597323/52‑1 (MQ=255) tAAACACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:828863/48‑1 (MQ=37) aaaCACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:564801/47‑1 (MQ=37) aaCACATTTAATAAA‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:428175/46‑1 (MQ=37) acacATTTAAT‑‑ATAAAATAAGGGCGGTGGCAAGATCGCCCttttt < 1:885015/45‑1 (MQ=25) acaTTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCttttt < 1:1510407/41‑1 (MQ=255) | GAAGTTCTGAAAAACGCGTAAACACATTTAAT‑‑A‑AAAA‑AAGGGCGGTCGCAAGATCGCCCTTTTT > W3110S.gb/715600‑715663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |