Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	25,091	T→A	C901S (TGC→AGC)	ileS →	isoleucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	25,091	0	T	A	100.0%	58.6 / NA	18	C901S (TGC→AGC)	ileS	isoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/0);  total (18/0)

AGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCA  >  W3110S.gb/25032‑25096                                                            |      agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:352580/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:881644/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:876859/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:779321/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:690651/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:666534/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:603447/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:389390/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:1056983/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:340146/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:22302/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:1820954/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:178218/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:1304812/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:1159007/1‑65 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcac   >  1:815363/1‑62 (MQ=255) agcagAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcac   >  1:1108585/1‑62 (MQ=255) agaagagCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGAGcaca  >  1:242296/4‑65 (MQ=255)                                                            |      AGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCA  >  W3110S.gb/25032‑25096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑