Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	993,079	C→A	T679N (ACT→AAT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	993,079	0	C	A	100.0%	248.0 / NA	66	T679N (ACT→AAT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/66);  total (0/66)

CGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATG  >  W3110S.gb/993026‑993090                                                      |            cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAATCGCAATCTGCGTAAtg  <  1:1472996/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtt  <  1:709069/65‑2 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1849212/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:454632/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:443312/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:431678/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:417382/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:378557/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:370536/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:340957/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:487702/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:183103/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1820011/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1759439/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1739164/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1735347/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1019235/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1706299/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:488070/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:501412/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:512062/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:547850/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:550298/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:55890/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:615028/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:677864/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:701652/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:821557/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:824015/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:826712/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:898230/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:993891/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1280388/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1032819/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1057512/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1075686/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1115504/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1151098/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1156678/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1163076/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1167915/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1195724/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1200818/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1228359/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1255458/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1259864/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1689974/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1319974/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1336621/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1623477/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1615424/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1586221/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1571021/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1551267/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:150139/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:149892/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1456514/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1412428/65‑1 (MQ=255) cgcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:132061/65‑1 (MQ=255)  gcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:502769/64‑1 (MQ=255)  gcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:109631/64‑1 (MQ=255)  gcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1338364/64‑1 (MQ=255)  gcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1721663/64‑1 (MQ=255)        tACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1569403/58‑1 (MQ=255)                     aCCGTGTTGAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:1567818/45‑1 (MQ=255)                             gAGCATGAAGATATTGCAAAACGCAATCTGCGTAAtg  <  1:989885/37‑1 (MQ=255)                                                      |            CGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATG  >  W3110S.gb/993026‑993090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑