Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,153,717	C→G	Q68E (CAG→GAG)	fabF →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,153,717	0	C	G	100.0%	200.1 / NA	54	Q68E (CAG→GAG)	fabF	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (54/0);  total (54/0)

GAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCA  >  W3110S.gb/1153659‑1153723                                                           |       gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAgcac   >  1:950154/1‑62 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAgcac   >  1:1443540/1‑62 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:271357/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:242486/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:316042/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:332842/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:337370/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:343142/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:395291/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:403526/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:4058/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:43307/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:464831/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:468753/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:509415/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:54987/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:598158/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:654343/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:701622/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:717710/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:758563/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:796161/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:800595/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:861114/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:880342/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:885461/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:915540/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1569147/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1068014/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1112448/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:114197/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1171102/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1199985/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1215691/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:122525/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:12551/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1462609/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1521066/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1529609/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1530601/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1030975/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1571257/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1623259/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1645412/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1745028/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1786399/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:179904/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1813471/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:1855301/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:186154/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:20212/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:22672/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:2314/1‑65 (MQ=255) gAAATTTGCTGGCTTAGTAAAGGATTTTAACAGTGAGGACATTATCTCGCGCAAAGAAGAGcaca  >  1:852467/1‑65 (MQ=255)                                                           |       GAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCA  >  W3110S.gb/1153659‑1153723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑