Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,389,988	C→T	intergenic (+13/+31)	tyrR → / ← tpx	DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,389,988	0	C	T	100.0%	194.5 / NA	52	intergenic (+13/+31)	tyrR/tpx	DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/0);  total (52/0)

CTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCAT  >  W3110S.gb/1389949‑1390012                                        |                         ctaaGTCAGATGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:241735/4‑64 (MQ=38) cTGAGTCAGAAGAAGCACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1286932/1‑64 (MQ=39) cTGAGTCAGAAGAAGAACGAAGGGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1755047/1‑64 (MQ=39) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATgg                    >  1:407922/1‑46 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATg                     >  1:1551217/1‑45 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATg                     >  1:1097847/1‑45 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGc                 >  1:98534/1‑49 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACTCCATCAGGCat  >  1:1268181/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACCCCATCAGGCa   >  1:586909/1‑63 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAgga    >  1:788804/1‑61 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:423320/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1738876/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:938993/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1762821/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:89997/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1819068/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1886165/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:85329/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:815874/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:419996/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:780460/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:55867/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:808694/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1642933/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:590414/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:651650/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:710699/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:747578/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1260215/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1055046/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1060022/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1063155/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1100128/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1103665/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1112536/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1123296/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1208562/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1222278/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1237430/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1244948/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:172444/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1262503/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1348629/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1394508/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1409812/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1416746/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1422378/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1505980/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1518386/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1615088/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1030751/1‑64 (MQ=255) cTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCat  >  1:1660600/1‑64 (MQ=255)                                        |                         CTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCAT  >  W3110S.gb/1389949‑1390012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑