Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,081,459	A→G	F36L (TTT→CTT)	yeeX ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,081,459	0	A	G	98.5%	251.1 / ‑4.2	68	F36L (TTT→CTT)	yeeX	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (0/67);  total (0/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCA  >  W3110S.gb/2081412‑2081497                                                |                                       cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTc                            <  1:573615/60‑1 (MQ=255)                       ttCTTACGACGGAAGAGACGAACAAGTTCCAGTACGGCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:273125/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGTCTTGGTAGTTTCCa  <  1:753236/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:462895/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:642832/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:626847/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:620399/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:61478/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:578424/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:564615/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:680107/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:407652/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:38764/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:366757/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:28637/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:269702/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1022903/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:653132/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:242924/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:689462/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:709431/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:718009/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:758981/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:764562/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:768306/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:805765/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:83771/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:838057/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:89826/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:936642/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:939914/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:941260/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:97762/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:124225/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1464340/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1444970/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1393874/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1331656/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1330301/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1320753/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1289660/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1256451/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:20167/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1113467/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1098329/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1094087/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:108900/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1074744/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1067998/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1038759/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:170591/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:227601/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:186841/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1838879/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1773092/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1735063/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1723276/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1668500/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1647012/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1639184/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1608189/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1557902/64‑1 (MQ=255)                       ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTACa  <  1:3771/64‑1 (MQ=255)                        tCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1655207/63‑1 (MQ=255)                        tCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1596770/63‑1 (MQ=255)                                 ggAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:650955/54‑1 (MQ=255)                                     cAGACGAACAAGTTCCAGTTCGTCCTGGAATGAATGCTTGGTAGTTTCCa  <  1:1511520/50‑1 (MQ=255)                                      aGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCa  <  1:1133422/49‑1 (MQ=255)                                                |                                       CTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCA  >  W3110S.gb/2081412‑2081497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑