Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,118,670	T→G	I199L (ATC→CTC)	wcaL ←	predicted glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,118,670	0	T	G	100.0%	133.1 / NA	36	I199L (ATC→CTC)	wcaL	predicted glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/0);  total (36/0)

CGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCC  >  W3110S.gb/2118649‑2118710                      |                                         cGCCCCTGCGCGATACGGCGAGTTTTTCCCTCGGGCAGc                         >  1:478317/1‑39 (MQ=37) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCCGCCTTCCGGcc  >  1:653811/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCgg    >  1:1406457/1‑60 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCgg    >  1:1526571/1‑60 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:528803/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:298992/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:299055/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:304096/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:317783/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:3188/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:347804/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:364772/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:385013/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1844648/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:532423/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:835291/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:847024/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:854508/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:945776/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1718878/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:115402/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1172563/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1369597/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1424458/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1434776/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:154552/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1626934/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1633061/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1097363/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1730839/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1731800/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1748143/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1749530/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1768582/1‑62 (MQ=255) cGCCCATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:1819226/1‑62 (MQ=255)  cgccATGCGCGATACGGCGAGTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGcc  >  1:309896/3‑61 (MQ=255)                      |                                         CGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCC  >  W3110S.gb/2118649‑2118710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑