Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,521,463	C→T	A606T (GCG→ACG)	yfeA ←	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,521,463	0	C	T	100.0%	337.3 / NA	95	A606T (GCG→ACG)	yfeA	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/95);  total (0/95)

CTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATG  >  W3110S.gb/2521411‑2521476                                                     |              ctgctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAt   <  1:1556315/65‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:428730/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:593245/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:589572/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:587146/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:56061/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:549836/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:543448/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:530467/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:524313/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:514207/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:448756/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:621370/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:37329/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:332257/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:296912/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:292313/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:246670/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:240690/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:237554/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1866874/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1849595/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1834551/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1005007/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:72270/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:979532/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:977579/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:920283/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:912447/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:865191/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:8542/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:83863/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:808299/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:768017/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:765389/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:75004/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1802927/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:711135/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:710458/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:709888/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:700634/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:694716/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:656470/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:653540/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:652627/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:642343/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:639585/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:117158/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1794247/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:128334/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1251581/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1225365/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1217983/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1204198/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1203964/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1197857/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1193213/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1187169/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:11797/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1290598/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1130260/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1113596/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1111656/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1102848/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:109150/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1070582/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1052354/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1031887/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:102531/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1021981/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1594259/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:175023/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1714308/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1696556/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1694966/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1690551/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1671207/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1652546/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:163372/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1610926/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1329034/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1584707/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:156511/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1559705/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1556964/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1519716/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1503584/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1496460/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1440136/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1347146/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1341971/64‑1 (MQ=255)   gctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGAGCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:476778/64‑1 (MQ=255)    ctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:227477/63‑1 (MQ=255)           cGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:762673/56‑1 (MQ=255)                 aaaaCGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtg  <  1:1065158/50‑1 (MQ=255)                                                     |              CTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATG  >  W3110S.gb/2521411‑2521476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑