Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,611,108 T→C F185L (TTC→CTC)  hyfR → DNA‑binding transcriptional activator, formate sensing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,611,1080TC100.0% 764.4 / NA 200F185L (TTC→CTC) hyfRDNA‑binding transcriptional activator, formate sensing
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/200);  total (0/200)

Only 100 of 200 total aligned reads displayed.

TCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAA  >  W3110S.gb/2611062‑2611154
                                              |                                              
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1648361/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1879733/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1873398/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1867660/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1862895/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1843048/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1715578/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1670511/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1655360/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:284693/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1642228/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1630991/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:159472/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1570972/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1549284/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1536804/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1527144/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1014464/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:307172/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:339183/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:387211/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:46246/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:477647/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:484720/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:548098/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:564424/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:697188/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:743000/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:819429/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:930704/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:942730/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:987326/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:991606/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1253693/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1524353/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1429480/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1118430/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1076945/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1296821/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1062107/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:141890/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1126188/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGGCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:760297/62‑1 (MQ=255)
 ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:796903/61‑1 (MQ=255)
 ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1044325/61‑1 (MQ=255)
       gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:573195/55‑1 (MQ=255)
        aTCTGATGGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1744057/54‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:706910/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1129726/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:406314/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:511240/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:529814/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1177029/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1183936/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:561910/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1111814/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:586470/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:601041/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:362027/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:77693/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:806042/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:896307/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:965837/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1036630/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1004534/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:316904/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1336180/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1282392/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1391056/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1426017/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1263026/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1228940/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1881736/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1591196/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:217117/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1447318/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:122691/53‑1 (MQ=255)
               cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:332779/53‑1 (MQ=255)
                gCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:252081/52‑1 (MQ=255)
                   gACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcat                                 <  1:1268982/43‑2 (MQ=39)
                   gACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa                           <  1:1378112/49‑1 (MQ=255)
                        cGCTCGGGAGATCCATCATTTTCTCGGTCTGGCTtcag                                 <  1:1050155/38‑1 (MQ=37)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:927322/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1601258/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:88549/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:867177/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1470892/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:166101/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:750055/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1788632/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:108713/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:656970/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:631658/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1801853/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1834326/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1274288/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1884137/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1134284/65‑1 (MQ=255)
                            cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1208792/65‑1 (MQ=255)
                                         atTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa  <  1:1611932/52‑1 (MQ=255)
                                              |                                              
TCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAA  >  W3110S.gb/2611062‑2611154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑