Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,966,285	T→C	E269G (GAG→GGG)	ptsP ←	fused PEP‑protein phosphotransferase (enzyme I) of PTS system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,966,285	0	T	C	100.0%	88.8 / NA	25	E269G (GAG→GGG)	ptsP	fused PEP‑protein phosphotransferase (enzyme I) of PTS system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/0);  total (25/0)

TCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCA  >  W3110S.gb/2966228‑2966291                                                          |       tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGCCCGTTTTTACCGCCCACCCTGCCa  >  1:524911/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGcc   >  1:1862285/1‑63 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGc    >  1:291764/1‑62 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:245735/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:934106/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:870448/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:734074/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:649751/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:643546/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:577170/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:567434/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:530499/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:458429/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:353168/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1090820/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:19186/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1787236/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1600771/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1591147/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1574931/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1457624/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1332457/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1283784/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1110484/1‑64 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCa  >  1:1091686/1‑64 (MQ=255)                                                          |       TCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCA  >  W3110S.gb/2966228‑2966291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑