Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	379,608	G→A	I153I (ATC→ATT)	yaiO ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	379,608	0	G	A	100.0%	201.8 / NA	55	I153I (ATC→ATT)	yaiO	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (55/0);  total (55/0)

CCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGATCACCGGGCCAGTATAGA  >  W3110S.gb/379564‑379627                                             |                    cccccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1180792/4‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTTTAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1442238/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATc                  >  1:877748/1‑48 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:695533/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:235754/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:236654/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:254410/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:256066/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:277846/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:436440/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:470146/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:507307/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:602839/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:632799/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:663087/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:693604/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1855260/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:729815/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:778943/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:843760/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:875319/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:888974/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:895603/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:90017/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:919593/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:940716/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:981868/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:989548/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1315460/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1065032/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1085950/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1137834/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1157403/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1158696/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1167201/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1183474/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1212290/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1213872/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1238460/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1249997/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1259798/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1314367/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1863453/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1316021/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1338213/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1381895/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1418382/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1521105/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1532345/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1580807/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1667687/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:1773511/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATaga  >  1:106405/1‑64 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATag   >  1:635721/1‑63 (MQ=255) ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTAATCACCGGGCCAGTATag   >  1:1651390/1‑63 (MQ=255)                                             |                    CCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGATCACCGGGCCAGTATAGA  >  W3110S.gb/379564‑379627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑