Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,031,910	A→G	N64D (AAC→GAC)	idi →	isopentenyl diphosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,031,910	0	A	G	100.0%	24.2 / NA	8	N64D (AAC→GAC)	idi	isopentenyl diphosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/8);  total (0/8)

ATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTG  >  W3110S.gb/3031858‑3031922                                                     |             attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:1536106/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:1632034/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:1775141/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:1807891/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:204249/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:345127/65‑1 (MQ=255) attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:942756/65‑1 (MQ=255)      ttACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg  <  1:1798476/60‑1 (MQ=255)                                                     |             ATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTG  >  W3110S.gb/3031858‑3031922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑