| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,228,312 | G→T | A257S (GCT→TCT) | ygjK → | predicted glycosyl hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,228,312 | 0 | G | T | 96.6% | 313.9 / 3.1 | 88 | A257S (GCT→TCT) | ygjK | predicted glycosyl hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base T (85/0); total (88/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGG > W3110S.gb/3228269‑3228366 | cGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAagag > 1:1135728/1‑64 (MQ=255)cGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAagag > 1:350322/1‑64 (MQ=255)cGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAagag > 1:1544320/1‑64 (MQ=255) tCTGCTGCCCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1056486/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGgc > 1:382386/1‑57 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGGGCGTCCgg > 1:1863116/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTc > 1:1546343/1‑62 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:501327/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:531031/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:496613/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:487479/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:473536/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:461383/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:424446/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:394196/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:369888/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:320718/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:319733/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:317248/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:220963/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1865024/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1843355/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1819104/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:716845/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:993358/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:987617/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:969638/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:941916/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:864140/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:803125/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:779177/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:764927/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:7465/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:530331/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:687193/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:666454/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:592900/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:591881/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:58147/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:57494/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:572048/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:571596/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1783305/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1184222/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1417206/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1345420/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1782580/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1304295/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1289340/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1276828/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1222821/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1219916/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1200600/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1343679/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1166109/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1165626/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:114262/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1113175/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1110732/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:109630/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1092398/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1050077/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1046688/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1459111/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1771110/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1471170/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1735168/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1666062/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1507280/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1517723/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1536579/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1719435/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1676234/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1555402/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1565415/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCgg > 1:1575354/1‑65 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1659269/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1043511/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:942082/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:487404/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1146668/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1161275/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:610360/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:351598/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:421589/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1291538/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1343630/1‑64 (MQ=255) tCTGCTGACCTCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCg > 1:1421818/1‑64 (MQ=255) | CGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGG > W3110S.gb/3228269‑3228366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |