Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,230,217	A→G	intergenic (+322/‑104)	ygjK → / → fadH	predicted glycosyl hydrolase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,230,217	0	A	G	100.0%	140.5 / NA	38	intergenic (+322/‑104)	ygjK/fadH	predicted glycosyl hydrolase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/0);  total (38/0)

TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAA  >  W3110S.gb/3230164‑3230276                                                      |                                                            tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTccccgc                                                      >  1:1347325/1‑58 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATcac                                                    >  1:1293298/1‑63 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:573671/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:1795219/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:206591/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:500365/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:555545/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:1377504/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:1225521/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:960920/1‑65 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACaa                                                  >  1:99455/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAACCCCCGCACTCCCCTTTTCCCTTTTCTcc                     >  1:306790/1‑47 (MQ=39)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCg                    >  1:1436020/1‑48 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTa     >  1:520293/1‑63 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg    >  1:1241605/1‑64 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg    >  1:344529/1‑64 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:804060/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:786025/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:778224/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1587347/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:844236/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:898423/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:423864/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1716486/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1699636/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1562879/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1551283/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1479604/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1459007/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1338453/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:133032/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1308990/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1291502/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1221407/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1083014/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1080592/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa   >  1:1020511/1‑65 (MQ=255)                                                ttttGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCC‑TTTCTCCGGCGACGGCTAAATTAGaa  >  1:1010542/1‑65 (MQ=255)                                                      |                                                            TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAA  >  W3110S.gb/3230164‑3230276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑