Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,383,928	C→T	A66T (GCG→ACG)	mdh ←	malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,383,928	0	C	T	100.0%	210.2 / NA	56	A66T (GCG→ACG)	mdh	malate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (56/0);  total (56/0)

GTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCT  >  W3110S.gb/3383877‑3383956                                                    |                             gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGTCTTCCAGCGTCGGAGTCGCATc                  >  1:950516/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCAt                   >  1:433883/1‑63 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1035238/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:361314/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1596473/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1563104/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1361709/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1231433/1‑64 (MQ=255) gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1008794/1‑64 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTGGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1131467/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGc                     >  1:1692815/1‑46 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCAt                   >  1:1588145/1‑48 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCtt                >  1:310497/1‑51 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCt                 >  1:1669234/1‑50 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCCCCAGAAAAACCt  >  1:1222744/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAg          >  1:141551/1‑57 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAccc   >  1:39314/1‑64 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:1143740/1‑64 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:1780366/1‑64 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:901684/1‑64 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:870583/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:287231/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:290239/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1194728/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1088813/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1074963/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:466374/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:476000/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:755899/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:52651/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:538157/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:865673/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:561466/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:566629/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:602146/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:695574/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1562877/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1253559/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1167856/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1409532/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1451108/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1497153/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1539017/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1553732/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1554999/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:260264/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1608812/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1612530/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1827202/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1875779/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1882048/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:23558/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:240561/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACAAGAAAAACCt  >  1:933830/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATATTCACCAGAAAAACCt  >  1:217765/1‑65 (MQ=255)                cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGCAGTCGCATCTTCACCAGAAAAACCt  >  1:175481/1‑65 (MQ=255)                                                    |                             GTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCT  >  W3110S.gb/3383877‑3383956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑