Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,608,041	C→T	L45L (CTG→TTG)	fadA →	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,608,041	0	C	T	100.0%	78.9 / NA	23	L45L (CTG→TTG)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/0);  total (23/0)

GCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTG  >  W3110S.gb/3608018‑3608080                        |                                        gCCTGCTGGCGCGTAACCCGGCGTTGGAAgcggcg                              >  1:1268504/1‑35 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGccc                          >  1:839675/1‑39 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcg                       >  1:1165578/1‑42 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcg                       >  1:829425/1‑42 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:985430/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1132721/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:811035/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:801678/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:571861/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:569807/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:544791/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:395814/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:291102/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1865577/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1673625/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1648199/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1488477/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1390863/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:1326306/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgtg  >  1:120417/1‑63 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgt   >  1:351770/1‑62 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgt   >  1:1344998/1‑62 (MQ=255) gCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCGcgacg                    >  1:72954/1‑45 (MQ=38)                        |                                        GCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTG  >  W3110S.gb/3608018‑3608080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑