Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,714,133	C→A	intergenic (+507/‑110)	gidB → / → atpI	methyltransferase, glucose‑inhibited cell‑division protein/ATP synthase, membrane‑bound accesory subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,714,133	0	C	A	100.0%	136.3 / NA	37	intergenic (+507/‑110)	gidB/atpI	methyltransferase, glucose‑inhibited cell‑division protein/ATP synthase, membrane‑bound accesory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/0);  total (37/0)

GTTTGAAATCACGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCT  >  W3110S.gb/3714122‑3714183            |                                                   gTTTGAAATCAAGGGGGCGCACCGTATAATTTGAcc                            >  1:554215/1‑36 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGAt                  >  1:749547/1‑46 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGc                >  1:804758/1‑48 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGcc   >  1:1208496/1‑61 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGcc   >  1:1649535/1‑61 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:708774/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:380898/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:464844/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:565956/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:611000/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:623680/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:639109/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:642334/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:114412/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:798074/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:847706/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:852858/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:954420/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:958881/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:262401/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1218409/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1225808/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1277653/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1314304/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1478671/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1561141/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1563198/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1567249/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1578939/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1616286/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1665140/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1755548/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:189710/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:192953/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:347689/1‑62 (MQ=255) gTTTGAAATCAAGGGGGCGCACCGTATAATTTGACCGCTTTTTAATGCTTGACTCTAAGc    >  1:987714/1‑60 (MQ=255) gTTTGAAATCAAGGCGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCt  >  1:1064403/1‑62 (MQ=255)            |                                                   GTTTGAAATCACGGGGGCGCACCGTATAATTTGACCGCTTTTTGATGCTTGACTCTAAGCCT  >  W3110S.gb/3714122‑3714183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑