Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,824,368 G→A pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 12.6 / NA 5pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/5);  total (0/5)

AGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATAC  >  W3110S.gb/3824335‑3824398
                                 |                              
aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc  <  1:110707/64‑1 (MQ=255)
aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc  <  1:1115202/64‑1 (MQ=255)
aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc  <  1:1398182/64‑1 (MQ=255)
aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc  <  1:274881/64‑1 (MQ=255)
aGGGATGGTAGCCGCAGTTTCTGTCGGTATAGTAAACGGTGAAGCAATTTGCGATCTGGAATAc  <  1:228300/64‑1 (MQ=255)
                                 |                              
AGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATAC  >  W3110S.gb/3824335‑3824398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: