Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,051,271	G→T	S12I (AGC→ATC)	ugpC →	glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,051,271	0	G	T	100.0%	171.0 / NA	46	S12I (AGC→ATC)	ugpC	glycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/46);  total (0/46)

GGCAGGACTGAAATTACAGGCAGTAACCAAAAGCTGGGATGGTAAAACCCAGGTCATTAAACCGC  >  W3110S.gb/4051239‑4051303                                 |                                 ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:52385/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1834506/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1838974/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1005120/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:190814/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:210134/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:295484/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:349852/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:371270/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:384947/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:186793/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:720531/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:741531/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:788774/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:788789/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:81235/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:828333/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:884649/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:942733/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:943551/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1084814/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1754425/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1714002/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1701992/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1102779/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:113128/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1653691/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1370842/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1528536/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1513401/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1420525/65‑1 (MQ=255) ggCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1779112/65‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAATATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:429384/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:415676/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1507172/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1449654/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1509763/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:306636/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:78964/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1346305/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:815176/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1214276/64‑1 (MQ=255)  gCAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:176608/64‑1 (MQ=255)   cAGGACTGAAATTACAGGCAGTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1423954/63‑1 (MQ=255)                       gTAACCAAAATCTGGGATGGTAAAACCCAGGTCATTAAACCGc  <  1:1697820/43‑1 (MQ=255)                          aCCAAAATCTGGGATTGTAAAACCCAGGTCATTAACCCGc  <  1:219949/40‑1 (MQ=37)                                 |                                 GGCAGGACTGAAATTACAGGCAGTAACCAAAAGCTGGGATGGTAAAACCCAGGTCATTAAACCGC  >  W3110S.gb/4051239‑4051303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑