Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,544,610	T→C	intergenic (‑427/‑1029)	yjhA ← / → fimB	N‑acetylnuraminic acid outer membrane channel protein/tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,544,610	0	T	C	100.0%	48.2 / NA	14	intergenic (‑427/‑1029)	yjhA/fimB	N‑acetylnuraminic acid outer membrane channel protein/tyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/0);  total (14/0)

TGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAA  >  W3110S.gb/4544590‑4544654                     |                                             tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaaca   >  1:1415422/1‑64 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1048744/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:126688/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1444651/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1496059/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1555376/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1641010/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:1823853/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:264515/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:656803/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:696922/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:715102/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:834623/1‑65 (MQ=255) tGACTATCTTGGTGCACCTTCATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa  >  1:928531/1‑65 (MQ=255)                     |                                             TGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAA  >  W3110S.gb/4544590‑4544654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑