Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,605,956 C→T V82I (GTT→ATT)  dnaT ← DNA biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,605,9560CT100.0% 484.7 / NA 128V82I (GTT→ATT) dnaTDNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (121/7);  total (121/7)

Only 100 of 128 total aligned reads displayed.

ATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGC  >  W3110S.gb/4605895‑4605998
                                                             |                                          
aTAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATggg                                         <  1:619698/65‑1 (MQ=255)
aTAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATggg                                         <  1:1752739/65‑1 (MQ=255)
aTAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATggg                                         <  1:1714718/65‑1 (MQ=255)
 tAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATggg                                         <  1:1333279/64‑1 (MQ=255)
  aaaaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATggg                                         <  1:249003/63‑1 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGTGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:279468/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCgg                             >  1:1433800/1‑37 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGAt                     >  1:59800/1‑45 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGCTCGTCCagaga  >  1:1410664/1‑63 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCagag   >  1:805259/1‑63 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCagag   >  1:308459/1‑63 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCaga    >  1:260077/1‑62 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCa      >  1:120967/1‑60 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:413186/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1871201/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:201833/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:48664/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:444494/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:214359/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:223165/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:440121/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:435109/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:234707/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:39622/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:379657/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:374313/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:245023/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:592363/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:297090/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:257673/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:855110/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:995870/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:982522/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:970703/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:964345/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:959420/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:929204/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:916693/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:88657/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:884078/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:877316/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:857016/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:525048/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:841742/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:780430/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:747079/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:737984/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:716375/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:682341/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:669060/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:636462/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1829135/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:560576/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1179253/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:137988/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1363428/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1360788/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1338793/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1329288/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1327302/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1316005/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1259251/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1237474/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1221629/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1214469/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1380170/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1178582/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1164453/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1164333/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1163159/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1140405/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1094498/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:109276/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1067984/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1045282/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1040692/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1038801/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1616837/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1856042/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1038756/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1802444/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1773454/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1760430/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1748273/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1741747/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1732579/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:168609/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1663598/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1620792/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1856523/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1570400/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1537430/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1508112/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1503998/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1477620/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1469065/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1458362/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1445867/1‑64 (MQ=255)
                                        aTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:1405243/1‑64 (MQ=255)
                                        aTTTCCCCACGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc  >  1:523864/1‑64 (MQ=255)
                                                             |                                          
ATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGC  >  W3110S.gb/4605895‑4605998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑