Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,286,268 (TAT)3→2 coding (398‑400/1293 nt) yjcF ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,286,2620T.100.0% 534.2 / NA 118coding (406/1293 nt)yjcFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (0/118);  total (0/118)
*W3110S.gb4,286,2630A.100.0% 534.2 / NA 118coding (405/1293 nt)yjcFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/118);  total (0/118)
*W3110S.gb4,286,2640T.100.0% 534.2 / NA 118coding (404/1293 nt)yjcFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (0/118);  total (0/118)

Only 100 of 118 total aligned reads displayed.

AAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAA  >  W3110S.gb/4286222‑4286293
                                        |||                             
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACTGTGACATTATTTATaaaa  <  1:1436963/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:328360/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:487504/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:455069/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:450232/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:43676/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:432981/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:423142/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:413428/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:412007/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:355878/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:355498/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:341367/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:500332/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:326878/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:318809/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:300612/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:285261/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:268383/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:261820/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:247805/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:235808/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:23160/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:729347/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:941203/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:939642/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:938501/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:924817/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:868121/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:861797/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:859425/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:827787/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:805447/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:740985/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1025161/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:727468/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:716730/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:711332/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:636358/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:628953/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:621490/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:57657/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:552120/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:545396/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:534582/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1194233/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1339963/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1336729/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1288260/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1248768/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:123376/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1229915/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1228054/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1227297/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1223594/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1203689/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:181095/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1193764/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1186439/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1174375/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1157181/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:113959/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1118791/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1103115/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1044293/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1025831/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1012116/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1371859/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1570134/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1574091/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1507960/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1477622/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1433074/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1409842/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1406565/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1363055/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1356396/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1352983/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:158710/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:175699/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:188398/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACTGTCTGTGGGATTTCAATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1093368/69‑1 (MQ=255)
aaaTCGTAACCACCATTTACGGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:560399/69‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:120025/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1057419/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:937882/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1102481/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:252576/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:290246/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:152892/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:709447/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:42767/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1323648/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:447608/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:42722/68‑1 (MQ=255)
 aaTCGTAACCACCATTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:435117/68‑1 (MQ=255)
         ccaccaTTTACTGTCTGTGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1548824/60‑1 (MQ=255)
          caccaTTTACTGTCTGTGGTATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:1303587/59‑1 (MQ=255)
                   ctgtctgtGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:255583/50‑1 (MQ=255)
                   ctgtctgtGGGATTTCGATAG‑‑‑TATTATGGACGGTGACATTATTTATaaaa  <  1:53868/50‑1 (MQ=255)
                                        |||                             
AAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAA  >  W3110S.gb/4286222‑4286293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑