| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,035,876 | (T)8→9 | intergenic (‑255/‑312) | yedR ← / → yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,035,868 | 1 | . | T | 99.4% | 597.5 / ‑3.8 | 162 | intergenic (‑247/‑320) | yedR/yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (95/66); total (96/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 167 total aligned reads displayed.
CGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGAT > W3110S.gb/2035831‑2035901 | cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTa > 1:1314389/1‑59 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATTTGCGCCt > 1:35672/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTgcg > 1:748803/1‑66 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGcc > 1:462357/1‑68 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGcc > 1:84261/1‑68 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGcc > 1:1111226/1‑68 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1002558/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:520432/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:489223/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:46177/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:404088/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:394728/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:328483/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:602470/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:234720/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:20524/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:201934/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:183472/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:178135/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:170718/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1580174/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:767495/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:9738/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:937867/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:90203/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:867709/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:854013/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:853899/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:830698/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:820274/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:52432/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:746570/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:731245/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:707049/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:671356/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:666354/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:617129/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:249077/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1008038/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1292057/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1267821/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1321289/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1333948/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1238157/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1364793/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1142529/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1132682/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1407417/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1438561/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1121540/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1067011/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:103387/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1444879/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1447799/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:145671/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1523292/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:150317/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1482072/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:1021222/1‑69 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑ttttttttt > 1:475294/1‑46 (MQ=255)cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt > 1:1526077/1‑46 (MQ=255)cGTCTTCTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt > 1:206903/1‑69 (MQ=255) cTTGTTTATATTTTTTGGGTCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1440303/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1244988/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:742859/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:697994/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1170362/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:677986/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:124704/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:875447/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:916097/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:569825/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:63848/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:625775/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1286267/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1561675/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1497642/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:213202/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:251964/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:257123/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:297781/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:407357/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:440143/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:583340/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:553118/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:49758/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1297415/69‑1 (MQ=255) cTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1350609/69‑1 (MQ=255) ttGTTTATATTTTTTGGGCCTGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:949945/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1036927/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:619055/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:885486/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:486628/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1402600/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1366820/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:116221/68‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:71824/68‑1 (MQ=255) tGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1054758/67‑1 (MQ=255) tGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1019463/67‑1 (MQ=255) gatgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:1564506/43‑1 (MQ=38) | CGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGAT > W3110S.gb/2035831‑2035901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |