Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,169,967 (T)7→8 intergenic (‑10/+159) yegZ ← / ← yegR ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,169,9601.T99.4% 632.4 / ‑3.5 173intergenic (‑3/+166)yegZ/yegRECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (0/172);  total (0/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 289 total aligned reads displayed.

GTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATA  >  W3110S.gb/2169894‑2169974
                                                                   |              
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACGCGGGCTTA‑tt              >  1:1494195/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:611396/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1402868/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:769526/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1429289/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1463500/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1479371/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:151208/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:736670/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:734519/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:664036/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:634762/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1455839/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:580188/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:542982/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:491458/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:280099/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:455916/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:454032/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:444979/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:411155/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:405535/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:379786/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:403211/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:786658/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:989178/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1192540/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:867974/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1221095/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:855041/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:830206/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1264978/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1386568/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:13107/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1321100/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1340140/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1304911/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1356333/1‑69 (MQ=255)
gtgtTTCCGTGTAAATATCTGCCAGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt              >  1:1028935/1‑69 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:518292/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:962938/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:947958/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:424940/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:939962/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:907677/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:904889/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:468514/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:480805/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:881752/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:532168/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:553386/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:574344/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:752039/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:593515/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:324645/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:847329/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:818478/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:80234/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1245713/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1591950/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1589521/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:157213/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1543536/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:14210/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:137835/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1301315/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1293550/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:367685/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1235073/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1230617/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1200191/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1183470/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1161507/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1140847/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1015372/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:262147/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:371104/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:360947/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:34746/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:337643/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1004014/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:291033/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1598935/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:252332/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:217020/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:208556/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:200359/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:186461/69‑1 (MQ=255)
             aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1605732/69‑1 (MQ=255)
              atatCTGCCCGCCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:433555/68‑1 (MQ=255)
              atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1437573/68‑1 (MQ=255)
              atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:309739/68‑1 (MQ=255)
              atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1097941/68‑1 (MQ=255)
              atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1081163/68‑1 (MQ=255)
               tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:696629/67‑1 (MQ=255)
                           cGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:674503/55‑1 (MQ=39)
                                    ggCTGATTGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:893986/46‑1 (MQ=37)
                                     gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:1189040/45‑1 (MQ=38)
                                               aaCTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:244719/35‑1 (MQ=25)
                                               aaCTCTGCTACACGGGCTTATTTTTTTTATGCATa  <  1:617401/35‑1 (MQ=25)
                                                                   |              
GTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATA  >  W3110S.gb/2169894‑2169974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑