| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,299,667 | (A)5→6 | coding (104/444 nt) | yhbP ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,299,662 | 1 | . | A | 78.6% | 697.8 / 192.9 | 238 | coding (109/444 nt) | yhbP | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (51/0); new base A (187/0); total (238/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Only 100 of 238 total aligned reads displayed.
CGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCCTGC > W3110S.gb/3299611‑3299705 | cgTGGCTCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:207530/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:99546/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1432244/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:139742/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:30066/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:400845/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:425689/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1237486/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1207438/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1195029/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1141922/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:483429/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:672814/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:738278/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:84874/1‑68 (MQ=255)cgTGGCGCGTTTTTTCTTCCGGCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCac > 1:1231745/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAgg > 1:869917/1‑39 (MQ=38) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAgc > 1:322777/1‑46 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAg > 1:1275409/1‑45 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAg > 1:203415/1‑45 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCa > 1:468016/1‑49 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCGCCAGAGTTCCCCTTCctgc > 1:134818/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGtt > 1:891656/1‑57 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGt > 1:1519298/1‑56 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTcc > 1:1077228/1‑59 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTc > 1:1014227/1‑58 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:648833/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:749841/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1093649/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:724286/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:698939/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:688273/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:109969/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:664448/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:659742/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:752824/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:643861/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:638473/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:629552/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:610025/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:596167/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:581191/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:571030/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1438070/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:889634/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1003665/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:972747/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:960349/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:94171/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:930330/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:914868/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:902939/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1027050/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:550951/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:881747/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1028658/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:85229/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:107005/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:82544/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:814741/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:807829/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:766268/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1568396/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:547510/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1340333/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1373640/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:196204/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:168703/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1602599/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1596310/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1577797/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:221436/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1559820/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1535031/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1413422/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1504399/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1497706/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1486083/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1474079/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1457203/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:424363/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:527625/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1127060/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1211722/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:459449/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:448650/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:446293/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1293574/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:254818/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:421582/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1307569/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:356072/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:342065/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:132554/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:312454/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:1331324/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:274809/1‑68 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctg > 1:963809/1‑67 (MQ=255) gTAGAAGGCGACTTTCTGCGCATCAAAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCct > 1:392180/1‑66 (MQ=255) gTAGAAGGCGACTTTCTGCGCATC‑AAAATAGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCctgc > 1:983398/1‑68 (MQ=255) | CGTGGCGCGTTTTTTCTTCCGTCAAAATGTAGAAGGCGACTTTCTGCGCATC‑AAAA‑AGATAAAAGGCATTAGCGCACCAGAGTTCCCCTTCCTGC > W3110S.gb/3299611‑3299705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |