Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	1847189	1847263	75	51 [0]	[0] 73	topB	DNA topoisomerase III

TCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCA  >  W3110S.gb/1847120‑1847188                                                                     | tCGCTCGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:476169/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:551595/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:299068/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:301737/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:33790/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:389364/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:411892/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:41558/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:507094/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:514221/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:525112/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:535608/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:535654/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:277818/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:553438/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:576281/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:577380/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:579767/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:596546/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:609046/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:613339/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:651147/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:70477/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:706920/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:799005/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:824187/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1329362/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1059773/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1068327/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1132102/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1145873/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1159943/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1186645/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:120926/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1216820/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1270195/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1287245/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1313755/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1034624/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1335160/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1346961/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1360330/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1360800/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1432273/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1471181/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1502693/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1561439/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:261406/1‑69 (MQ=255) tCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:267298/1‑69 (MQ=255) tCGCACAGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:2146/1‑69 (MQ=255) acGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCa  >  1:1163880/2‑69 (MQ=255)                                                                     | TCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCA  >  W3110S.gb/1847120‑1847188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑