| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,950,447 | 1 | . | G | 41.9% | 142.2 / inf | 325 | coding (292/1107 nt) | bcsZ | endo‑1,4‑D‑glucanase |
| Reads supporting (aligned to +/- strand): ref base . (76/113); new base G (136/0); total (212/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-36 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 328 total aligned reads displayed.
TGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTG > W3110S.gb/3950390‑3950482 | tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:518517/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1552017/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1578071/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:17426/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:62869/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1389597/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:946261/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:216482/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:22643/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:24191/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:960276/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1300740/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1277798/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:273976/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:573835/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1069943/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:1076407/69‑1 (MQ=255)tGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:836096/69‑1 (MQ=255) ggACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:712025/68‑1 (MQ=255) cAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCaagaaa < 1:258284/58‑1 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAg > 1:1305645/1‑62 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGaa > 1:162169/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGaa > 1:842916/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGaa > 1:34729/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGaa > 1:1430100/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1562924/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:540680/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:648634/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:168040/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:464514/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1469600/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:601960/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:586287/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:457577/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:429871/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:895093/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:553657/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:374875/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:280937/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1526956/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1039562/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1054916/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1126998/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:114827/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1178072/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:790672/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1213128/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:888591/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:701833/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1337466/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1357041/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:664128/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1370092/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:929563/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1407057/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1419542/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGGCAAGAAAGAGAACAGTAAGCGGGAAg > 1:565420/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑ggg > 1:867533/1‑41 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGt > 1:846389/1‑62 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:397910/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:1452238/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAg > 1:447771/1‑68 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:554865/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:775894/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:193827/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:670133/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:726060/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:682694/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1367109/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1001710/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1087352/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1109570/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1204375/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:975430/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1239462/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1251547/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1260620/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1281373/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:497579/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1477113/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1542844/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1594533/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:210959/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:223161/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:291121/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:912065/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:339017/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:908506/1‑69 (MQ=255) tCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGA‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGt > 1:1192277/1‑69 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:980991/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:815358/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:769397/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:421264/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:391699/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:334930/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:1441550/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:1385669/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:1312650/68‑1 (MQ=255) gTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:1215753/68‑1 (MQ=255) cccGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTg < 1:1018028/48‑1 (MQ=255) | TGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGT‑GGGGCAAGAAAGAGAACAGTAAGTGGGAAGTGCTG > W3110S.gb/3950390‑3950482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |