Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,244,9230C.71.8% 370.8 / 265.7 195coding (924/1476 nt)xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base C (0/55);  new base . (140/0);  total (140/55)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

AACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAC  >  W3110S.gb/4244878‑4244956
                                             |                                 
aaCTCCGACAATTATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgc           >  1:115462/1‑67 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGccag                                 >  1:949373/1‑46 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGccag                                 >  1:1512342/1‑46 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:411502/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:596637/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:579820/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:54854/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:543992/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:53450/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:52787/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:511347/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:484960/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:46732/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:425456/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:643050/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:389569/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:365756/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:348769/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:308719/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:248609/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:231438/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:218085/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:769549/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:979493/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:966853/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:93305/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:920522/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:893797/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:84600/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:84274/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:835201/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:805463/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:635299/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:74915/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:724437/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:716432/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:702224/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:684190/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:668347/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:656619/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1010307/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:162878/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1470420/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1460735/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1438071/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1381517/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1088856/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1345705/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1138979/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1331055/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1405741/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1326614/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:131706/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1290828/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1259168/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1243891/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1198387/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1225332/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1046706/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1009405/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:102131/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1561174/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:155288/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1045443/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1544819/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1485082/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1064541/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1537259/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1526987/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1520034/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgcg          >  1:1496073/1‑68 (MQ=255)
aaCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGG‑‑‑CCAGCGTTTTGAACACTTCCGgc           >  1:1186678/1‑67 (MQ=255)
          ataatGGTGTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1320916/69‑1 (MQ=255)
          ataatGGTCTGCAACATCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:26698/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:82375/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:911120/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1134233/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1070092/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:801335/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:788838/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:77854/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:608417/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:861416/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1369934/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:182655/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:294795/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:326856/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:336452/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:360701/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:369945/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:375109/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:74539/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1357607/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:579898/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:587962/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:640678/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1306718/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1273814/69‑1 (MQ=255)
          ataatGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:713538/69‑1 (MQ=255)
                           cgcgATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc  <  1:1575680/52‑1 (MQ=255)
                                             |                                 
AACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAC  >  W3110S.gb/4244878‑4244956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑