| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,677,208 | 1 | . | C | 24.1% | 490.2 / 265.7 | 216 | coding (867/1389 nt) | pntB | pyridine nucleotide transhydrogenase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base . (111/53); new base C (52/0); total (163/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 217 total aligned reads displayed.
CACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCC > W3110S.gb/1677148‑1677249 | cacaGAATGGGAGTTTTTCAGCAGTTCCGCTGTCACTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:424763/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTGTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1059835/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTTA‑CCCACTTc > 1:435425/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCa‑cacac > 1:696431/1‑66 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACtt > 1:1342409/1‑68 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACtcc > 1:1037966/1‑67 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACt > 1:1002046/1‑67 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:332155/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:445619/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:405374/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:389241/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:358745/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:349339/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:344700/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:550955/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:301176/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:242010/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:226180/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:212938/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:174277/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1553962/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:790008/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:970408/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:960196/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:953846/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:89491/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:885161/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:86021/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:835644/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:828026/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:532418/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:777420/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:683611/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:670208/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:665671/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:635683/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:607665/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:275513/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1402161/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1279253/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1285784/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1312653/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1380366/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1028793/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1119223/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:120655/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1489718/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:1193726/1‑69 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTAA‑CCCa > 1:935370/1‑65 (MQ=255)cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTG‑GGTGATTTCGCGGTGCTCA‑CCCACTTc > 1:798069/1‑68 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:726412/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:42900/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1184336/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1273712/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:689935/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:87982/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:514805/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1143622/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1168994/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:820444/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:622033/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:63206/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1287892/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1549169/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:314646/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:157759/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1596761/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1020853/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1527188/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:257911/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1449339/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:1482397/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:96036/69‑1 (MQ=255) aGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:190155/69‑1 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCGCTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:142834/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1018130/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:80697/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1128917/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1077658/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:951574/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1042330/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1068680/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1278568/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:16332/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1458535/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:277034/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1387683/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1345995/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1327190/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:745222/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:484852/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:127/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:124898/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:592873/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1230583/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1216891/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGcc > 1:1198966/1‑67 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGc > 1:282140/1‑66 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCACCCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGc > 1:645792/1‑66 (MQ=255) ttctGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGc < 1:918127/44‑1 (MQ=255) | CACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCA‑CCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCC > W3110S.gb/1677148‑1677249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |