| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,554,749 | A→G | D268D (GAT→GAC) | adhP ← | alcohol dehydrogenase, 1‑propanol preferring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,554,749 | 0 | A | G | 96.7% | 740.1 / 7.7 | 209 | D268D (GAT→GAC) | adhP | alcohol dehydrogenase, 1‑propanol preferring |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base G (0/202); total (7/202) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
Only 100 of 209 total aligned reads displayed.
GCGTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACAC > W3110S.gb/1554694‑1554792 | gcgTGCCGACCAGCGAACCGACCACTTCAATACCATTCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1070357/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:375933/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:585298/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:577383/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:555065/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:54744/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:505915/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:49856/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:485086/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:453026/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:434217/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:419123/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:393486/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:618146/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:35100/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:342726/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:331408/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:310278/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:300196/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:276404/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:264912/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:101763/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:248319/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:233411/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:821916/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:989847/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:971432/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:962071/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:954545/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:9446/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:921897/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:905013/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:883703/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:877618/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:863152/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:857558/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:604387/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:799172/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:737931/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:727025/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:713673/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:71186/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:701571/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:684089/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:664790/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:653256/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:253006/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1234141/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1381852/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1337195/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:133166/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:131323/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1300815/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1281498/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1279551/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1269625/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1263516/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1253319/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1247192/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1367856/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1223343/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1190698/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1162476/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1147007/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1122291/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1101110/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1083479/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1077065/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1024693/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1020691/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1511466/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:191729/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:185549/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:165176/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1603826/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1600992/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1591243/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1581079/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1573562/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1532052/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1524186/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1401430/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1496412/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1407217/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1474025/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1465593/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1437983/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1418751/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGACGTCCAGGCTCATAg < 1:149070/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGGGGGATGTCCAGGCTCATAg < 1:469926/69‑1 (MQ=255)gcgTGCCGACCAGCGAACCAACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:408145/69‑1 (MQ=255) cgTGCCGTCCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:134307/68‑1 (MQ=255) cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGTTGTCCAGGCTCATAg < 1:1211402/68‑1 (MQ=255) cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:381966/68‑1 (MQ=255) cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:563345/68‑1 (MQ=255) cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1055911/68‑1 (MQ=255) gTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:763122/67‑1 (MQ=255) ccAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:312583/60‑1 (MQ=255) tACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCcggcgg > 1:589859/1‑49 (MQ=255) tACCATACAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAacac > 1:216/1‑69 (MQ=255) | GCGTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACAC > W3110S.gb/1554694‑1554792 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |