Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 118,844 C→A L38M (CTG→ATG)  ampD → N‑acetyl‑anhydromuranmyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb118,8440CA100.0% 252.3 / NA 68L38M (CTG→ATG) ampDN‑acetyl‑anhydromuranmyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/68);  total (0/68)

GCACAATATTAGCCTGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTA  >  W3110S.gb/118831‑118898
             |                                                      
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAATTa  <  1:1080824/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:391168/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:604828/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:511825/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:511439/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:469711/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:434133/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:416999/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:414820/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:404866/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:656291/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:358856/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:342852/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:329167/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:294334/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:274047/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1017804/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:628147/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:26593/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:657769/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:670598/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:682809/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:704191/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:711300/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:774877/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:787885/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:801812/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:856182/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:866454/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:874017/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:875434/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:967409/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:970146/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1221886/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:242973/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1444956/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1436670/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1408906/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1347877/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1344807/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1334509/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1305747/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1468668/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1209468/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1207128/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1119548/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1089882/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1060799/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:105735/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1051358/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1585113/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:210548/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:20796/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:185701/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:180130/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1604832/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1596451/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1481520/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1581015/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1533498/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1528955/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1517550/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:150490/68‑1 (MQ=255)
gCACAATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1498885/68‑1 (MQ=255)
gCACAATATCAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:94266/68‑1 (MQ=255)
 cacaATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:80503/67‑1 (MQ=255)
 cacaATATTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:517792/67‑1 (MQ=255)
     atatTAGCATGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTa  <  1:1526988/63‑1 (MQ=255)
             |                                                      
GCACAATATTAGCCTGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTA  >  W3110S.gb/118831‑118898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑