Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,876,827	G→A	S155F (TCT→TTT)	yeaM ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,876,827	0	G	A	100.0%	320.1 / NA	87	S155F (TCT→TTT)	yeaM	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (87/0);  total (87/0)

CACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAG  >  W3110S.gb/1876799‑1876867                             |                                         caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTg                             >  1:215671/1‑42 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGTGGGAg  >  1:830085/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg     >  1:1571448/1‑66 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:653167/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:202755/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:262434/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:311239/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:345958/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:44084/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:49193/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:501013/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:530229/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:543656/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:555273/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:474372/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:689742/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:743355/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:752005/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:783969/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:786367/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:797774/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:837610/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:845871/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:959286/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:983806/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:1603703/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:1147352/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:132743/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:1072599/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:125165/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa   >  1:1218346/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:721311/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:721100/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:72368/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1212887/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:744340/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1195888/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:771646/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1604050/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:774346/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:775447/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1184660/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:654255/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1112954/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:111251/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:109182/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:844110/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:859155/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:862577/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:863571/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:882035/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:929583/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:944777/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:956369/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1050648/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:960984/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1021981/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:432453/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:161715/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:16839/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1587188/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:154542/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:231572/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:259013/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1523205/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:29996/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1498503/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:351313/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:35663/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:377925/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1225850/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:138967/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:463879/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:101061/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:476648/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:483250/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1326415/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:504181/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:511609/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1286085/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:1229725/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:588908/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg  >  1:62841/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGTTGTGGATGCTGAGGGAg  >  1:651407/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTGATGTGGCTGCTGAGGCAg  >  1:87743/1‑69 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTGTTCTTGTGGCTGCTGAGGGa   >  1:1405609/1‑68 (MQ=255) caccaTCGTGCGGATTTTAGGATGAGAAAAAACGGGCAGGTGCAGCTCTTGTTGTGGCTGCTGAGGGAg  >  1:502504/1‑69 (MQ=255)                             |                                         CACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAG  >  W3110S.gb/1876799‑1876867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑