Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,128,322	C→T	R13H (CGC→CAC)	nudD ←	GDP‑mannose mannosyl hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,128,322	0	C	T	100.0%	107.2 / NA	30	R13H (CGC→CAC)	nudD	GDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/0);  total (30/0)

GACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAA  >  W3110S.gb/2128288‑2128337                                   |                gACAATGAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:762494/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTAc          >  1:664153/1‑42 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGt       >  1:116184/1‑45 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:453943/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:964631/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:867413/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:807424/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:773944/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:67192/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:620611/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:57592/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:567268/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:542125/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:541102/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:535295/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:531172/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1050849/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:367746/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:230403/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:228598/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1583563/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1566458/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1560020/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1534448/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:141683/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1401469/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1384609/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:115640/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTg      >  1:1074655/1‑46 (MQ=255) gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGTGCACTACCGTGGCaa  >  1:214862/1‑50 (MQ=255)                                   |                GACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAA  >  W3110S.gb/2128288‑2128337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑