Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,445,099 C→A A156A (GCG→GCT)  yfcJ ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,445,0990CA100.0% 929.8 / NA 248A156A (GCG→GCT) yfcJpredicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/248);  total (0/248)

Only 100 of 248 total aligned reads displayed.

CGAGGGCACCGTAAATCGCCATTCCGTTCCATGACATCACTTTGCCAGAGTGTTTTGGCCCTACGATGC  >  W3110S.gb/2445083‑2445151
                |                                                    
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:490408/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:649388/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:635360/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:632785/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:616822/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:609169/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:600446/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:575435/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:558303/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:545665/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:534093/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:52638/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:654999/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:47305/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:430415/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:413166/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:37868/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:355335/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:346294/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:330798/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:287138/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:276896/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1000769/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:263913/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:834594/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:991613/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:978932/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:937794/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:923379/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:90735/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:906302/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:890916/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:860171/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:85627/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:851255/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:836103/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:274046/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:823975/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:820439/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:810531/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:793716/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:767907/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:748145/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:739399/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:728689/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:709315/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:69433/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1171597/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:137151/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1358903/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1355956/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:134359/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1315487/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1285451/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1276340/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1258256/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1242810/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1228670/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1213431/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1193528/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1393768/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1158527/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1137575/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1120737/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1116495/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:109624/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1090102/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1071009/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1069507/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1050628/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1047424/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1025786/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:101637/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:233705/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1606817/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:160431/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1592128/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:182362/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:20196/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:211661/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1558667/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1551376/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1540553/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1509706/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1495167/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1477852/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1469802/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:2429/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1446941/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1425737/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:140793/69‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:254141/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:187473/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:39008/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1561906/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:460956/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:502635/68‑1 (MQ=255)
  aGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:326251/67‑1 (MQ=255)
       aCCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1566782/62‑1 (MQ=255)
             aaTAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:665886/56‑1 (MQ=255)
                |                                                    
CGAGGGCACCGTAAATCGCCATTCCGTTCCATGACATCACTTTGCCAGAGTGTTTTGGCCCTACGATGC  >  W3110S.gb/2445083‑2445151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑