Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,459,445	C→A	V335F (GTC→TTC)	yfcU ←	predicted export usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,459,445	0	C	A	100.0%	177.2 / NA	48	V335F (GTC→TTC)	yfcU	predicted export usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/48);  total (0/48)

TACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATCA  >  W3110S.gb/2459436‑2459504          |                                                            tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:591458/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:100649/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:232168/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:280924/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:329477/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:331810/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:346357/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:379850/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:393584/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:409886/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:533235/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:568660/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:222930/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:599593/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:64853/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:765179/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:842345/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:864867/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:8866/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:888806/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:892339/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:920910/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:964838/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:995159/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1270062/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1035959/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1069639/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:112163/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1128971/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:114878/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1182051/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1209569/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:121442/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1219504/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1229010/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:173216/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:131541/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1353531/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1395789/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1398487/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1429499/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1449102/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1481853/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1501969/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1519756/69‑1 (MQ=255) tACCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1525498/69‑1 (MQ=255)  aCCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:934509/68‑1 (MQ=255)  aCCGGAGAAGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATca  <  1:1598612/68‑1 (MQ=255)          |                                                            TACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTGCGTTTCGTAAATCA  >  W3110S.gb/2459436‑2459504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑