Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 230,040 C→A R281L (CGC→CTC)  yafC ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb230,0400CA100.0% 930.0 / NA 253R281L (CGC→CTC) yafCpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (185/68);  total (185/68)

Only 100 of 254 total aligned reads displayed.

TGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGT  >  W3110S.gb/229978‑230064
                                                              |                        
tgtcAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:54798/4‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGcgcg                       >  1:712094/1‑66 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:410363/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:561254/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:540246/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:527278/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:513843/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:46656/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:446275/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:436773/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:420592/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:581121/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:386748/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:382987/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:3739/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:354878/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:323663/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:308168/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:269306/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:235163/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:233145/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:214371/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:84768/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:986367/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:974556/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:961463/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:932060/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:881467/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:87587/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:864716/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:860464/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:848231/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1011400/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:840945/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:825791/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:77714/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:738005/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:717731/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:696327/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:679529/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:639265/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:59618/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1131660/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1381611/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1356340/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1279910/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1271371/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:125114/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1243974/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1212582/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1167069/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1158577/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1140400/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:178258/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1120157/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1112221/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1089727/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1086748/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1058808/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1048907/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1042350/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1011934/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1002732/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1458959/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1503193/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1509638/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1553280/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1460550/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1424680/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:162998/1‑68 (MQ=255)
tGACAGCTCCTCCGGGAGCTGCTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa                     >  1:1392560/1‑68 (MQ=255)
                               ctctctAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:176824/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:278436/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:9030/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:290465/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1565241/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:753826/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:936747/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:348763/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1541435/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1530556/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:381698/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:203095/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:636877/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:489238/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:545167/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:133399/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1288323/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:59373/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:618393/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:763387/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:123250/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1470494/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1192746/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:724192/56‑1 (MQ=255)
                               cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:726875/56‑1 (MQ=255)
                                tCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:853543/55‑1 (MQ=255)
                                tCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1174169/55‑1 (MQ=255)
                                             cGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:1438987/42‑1 (MQ=255)
                                                tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt  <  1:885621/39‑1 (MQ=255)
                                                              |                        
TGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGT  >  W3110S.gb/229978‑230064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑